Table 2.

Plasmids used in this study

PlasmidGenotype and featuresReference or source
pAB13Tn903dIIlacZ-containingEcoRI fragment from LELA1012 in pBSK KS+ 28
pAB14Tn903dIIlacZ-containingEcoRI fragment from LELA1566 in pBSK KS+ 28
pBluescript II KS (+) oriR (f1) MCS oriR (ColE1), Apr Stratagene
pBC SK (+) oriR (f1) MCS oriR (ColE1), Cmr Stratagene
pBR322 oriR (ColE1), AprTcr New England Biolabs
pCR2.1 oriR(f1) MCS oriR (ColE1), Apr Kmr Invitrogen
pMMB207 oriR (RSF1010), Cmr 25
pMMB207αbpMMB207 MCS, Cmr 30
pMMB207αb::GmpMMB207 MCS, Gmr This study
pMW100 icmEGCDJB-, tphA-, andicmF-containing fragment in pMMB207This study
pMW150Tn903dIIlacZ-containingEcoRI fragment from LELA3563 in pMMB207This study
pMW152Tn903dIIlacZ-containingEcoRI fragment from LELA1223 in pMMB207This study
pMW318Tn903dIIlacZ-containingEcoRI fragment from LELA1996 in pMMB207This study
pMW320Tn903dIIlacZ-containingEcoRI fragment from LELA1506 in pMMB207This study
pMW4201,864-bp icmJ-containing fragment in pBSK KS+This study
pMW4242,892-bp icmGCD-containing fragment in pBSK KS+This study
pMW5253,257-bpHindIII fragment from pMW100 in pBSK KS+This study
pMW528 icmB-containing fragment in pBSK KS+This study
pMW560 icmB-containing fragment in pBC SK+This study
pMW564897-bp icmC-containing fragment in pCR2.1This study
pMW565811-bpicmD-containing fragment in pCR2.1This study
pMW5661,495-bp icmG-containing fragment in pCR2.1This study
pMW5762,004-bp tphA-containing fragment in pUC18This study
pMW582pMW424 with aKpnI site at bp 2993This study
pMW5842,540-bpicmGCD-containing fragment in pUC18This study
pMW587pMW420 containing ΔicmJ with aSalI site at bp 2993This study
pMW589pMW576 containing ΔtphA with a SalI site at bp 6862This study
pMW591pMW584 containing ΔicmGwith a SalI site at bp 995This study
pMW593pMW584 containing ΔicmC with aSalI site at bp 1831This study
pMW596pMW589 with Km cassette inserted in ΔtphA This study
pMW598pMW591 with Km cassette inserted in ΔicmG This study
pMW600pMW593 with Km cassette inserted in ΔicmC This study
pMW6021,331-bpHindIII-BamHI fragment from pMW587 in pUC18This study
pMW6042,540-bp icmGCD-containing fragment in pMMB207This study
pMW606pMW602 with Kmr cassette inserted in ΔicmJ This study
pMW616ΔtphA::Kmr in pLAW344This study
pMW618ΔicmG::Kmr in pLAW344This study
pMW620ΔicmC::Kmr in pLAW344This study
pMW622ΔicmJ::Kmr in pLAW344This study
pMW6801,864-bp icmJ-containing fragment in pBC, opposite orientation to PlacUV5 This study
pMW6811,864-bpicmJ-containing fragment in pBC, same orientation to PlacUV5 This study
pMW728897-bpicmC-containing fragment in pMMB207αb, same orientation to Ptac This study
pMW730897-bpicmC-containing fragment in pMMB207αb, opposite orientation to Ptac This study
pMW734811-bpicmD-containing fragment in pMMB207αb, same orientation to Ptac This study
pMW736811-bpicmD-containing fragment in pMMB207αb, opposite orientation to Ptac This study
pMW7411,495-bpicmG-containing fragment in pMMB207αb, same orientation to Ptac This study
pMW7431,495-bpicmG-containing fragment in pMMB207αb, opposite orientation to Ptac This study
pMW7901,894-icmJ-containing fragment in pMMB207αb::Gm, opposite orientation to Ptac This study
pLB41 HindIII fragment containing Gmr in pBR322L. Babiss and D. Figurski
pUC18 oriR(ColE1) MCS, Apr New England Biolabs