Table 1.

Bacterial strains used

NameComment(s)Reference
S. typhimurium F98 (Nalr)Wild type 44
S. typhimurium F98 (Spcr)Wild type 44
S. typhimurium (Nalr) Tn5-TC1 mutants
 17/12/90-117 rfbB::Tn5-TC1; no O antigenThis work
 14/12/90-201 rfbB::Tn5-TC1; no O antigenThis work
 30/11/90-85 rfbK::Tn5-TC1; no O antigenThis work
 12/6/91-86 rfaK::Tn5-TC1; no O antigenThis work
 7/12/90-66No O antigenThis work
 13/2/91-163No O antigenThis work
 5/6/91-184No O antigenThis work
 30/11/90-93bNo O antigenThis work
 30/11/90-94No O antigenThis work
 14/12/90-7 rfaY::Tn5-TC1This work
 14/12/90-243 dksA::Tn5-TC1This work
 14/12/90-43 dksA::Tn5-TC1This work
 21/12/90-82 dksA::Tn5-TC1This work
 21/12/90-83 dksA::Tn5-TC1This work
 14/12/90-19 hupA::Tn5-TC1This work
 8/5/91-309 sipC::Tn5-TC1This work
 6/2/91-62 clpB::Tn5-TC1This work
 10/4/91-86 ptsC::Tn5-TC1This work
 14/12/90-14 ptsC::Tn5-TC1This work
S. typhimurium F98 (Spcr) transductants
 T14/12/90-7iTransductant derived from 14/12/90-7This work
 T14/12/90-7iiTransductant derived from 14/12/90-7This work
 T14/12/90-7iiiTransductant derived from 14/12/90-7This work
 T14/12/90-7ivTransductant derived from 14/12/90-7This work
 T14/12/90-243Transductant derived from 14/12/90-243This work
 T14/12/90-19Transductant derived from 14/12/90-19This work
 T8/5/91-309Transductant derived from 8/5/91-309This work
 T6/2/91-62Transductant derived from 6/2/91-62This work
 T10/4/91-86Transductant derived from 10/4/91-86This work
S. typhimurium F98 (Nalr) defined mutants
rfbK Kma insertion inrfbK This work
dksA Km insertion indksA This work
hupA Km insertion inhupA This work
sipC Km insertion insipC This work
ptsC Km insertion inptsC This work
E. coli strains
 SY327λpir 29
 SM10λpir 41
  • a Km, kanamycin.