TABLE 1.

L. pneumophila strains used in this study

StrainGenotypeReference
130bWild type; virulent, serogroup 1 strain29
NU247Mini-Tn10 phoA (Kmr) inserted into the mip gene of 130b3
NU253Mini-Tn10 phoA (Kmr) inserted into the surA gene of 130b3
AA108726-bp deletion in the mip gene of 130b44
AA109Intact mip reintroduced into the chromosome of AA10844
NU201Spontaneous streptomycin-resistant 130b19
NU203Kmr cassette inserted into the mip gene of NU20120
NU268Kmr cassette inserted into the plcA gene of 130b4
NU275Kmr cassette inserted into the lspF gene of 130b70
NU317Kmr cassette inserted into the plcA gene of AA108This study