TABLE 2.

Summary of immunofluorescence detection of 50 C. trachomatis proteins in infected cells using mouse anti-fusion protein antibodies in comparison with previously known information

Location of protein and ORFPrevious evidenceaComputer predictionb
BanToh
Inclusion membrane
    CT089IM+
    CT115IM+++
    CT116IM+++
    CT117IM+++
    CT118IM+++
    CT119IM+++
    CT147IM++
    CT223IM+++
    CT225++
    CT226IM+++
    CT228++
    CT229IM+++
    CT232IM+++
    CT233IM+++
    CT249IM++
    CT288IM+++
    CT358++
    CT440++
    CT442IM++
    CT529IM+
    CT618IM++
    CT813IM+++
Intrainclusion
    CT058++
    CT192++
    CT195++
    CT383++
    CT484IB+++
    CT565+
    CT850+
Undefinedc
    CT005+
    CT006+
    CT036++
    CT101+
    CT134+
    CT135+
    CT164+
    CT179+
    CT196++
    CT214++
    CT222+
    CT224++
    CT227++
    CT300++
    CT345++
    CT357++
    CT365+
    CT449++
    CT483++
    CT728+
    CT789+
  • a Previous experimental evidence for intracellular location of the 50 proteins is listed in this column. Previously described proteins and the references in which their localizations are described are CT089 (14); CT115 to -119 (35); CT147 (6); CT223, -229, -233, -288, -442, and -484 (2); CT226 (38); CT229 (30); CT232 (IncB) and CT233 (IncC) (3); CT249 (22); CT442 (44); CT529 (15); CT618 (42); and CT813 (8). IM+, positive localization in the inclusion membrane; IB+, positive localization inside the inclusions; −, published experimental evidence for the intracellular location of the corresponding proteins is lacking.

  • b Ban and Toh stand for references 2, Bannantine et al., and 49, Toh et al., respectively. +, positive prediction for inclusion membrane localization; −, negative prediction for inclusion membrane localization.

  • c No specific signals were detected using the corresponding antibodies under the conditions in which the experiments were carried out.