TABLE 1.

Summary of anti-fusion protein antibody reactivity

AntibodyaReactivity with tagged fusion protein in indicated assayb
RFPGST (WB)
IFAWB
Anti-CT005+ND+
Anti-CT006++
Anti-CT036+ND+
Anti-CT058+ND+
Anti-CT089+ND+
Anti-CT101+ND+
Anti-CT115+ND+
Anti-CT116+ND+
Anti-CT117+ND+
Anti-CT118+ND+
Anti-CT119+ND+
Anti-CT134+c
Anti-CT135+ND+
Anti-CT147+ND+
Anti-CT164++
Anti-CT179+ND+
Anti-CT192+ND+
Anti-CT195+ND+
Anti-CT196++
Anti-CT214+ND+
Anti-CT222+ND+
Anti-CT223+ND+
Anti-CT224+ND+
Anti-CT225+ND+
Anti-CT226+ND+
Anti-CT227+c
Anti-CT228+ND+
Anti-CT229+ND+
Anti-CT232+ND+
Anti-CT233+ND+
Anti-CT249+ND+
Anti-CT288+ND+
Anti-CT300++
Anti-CT345+ND+
Anti-CT357+ND+
Anti-CT358+ND+
Anti-CT365+c
Anti-CT383+ND+
Anti-CT440+ND+
Anti-CT442+ND+
Anti-CT449+ND+
Anti-CT483+c
Anti-CT484+ND+
Anti-CT529+ND+
Anti-CT565+ND+
Anti-CT618+ND+
Anti-CT728+ND+
Anti-CT789+c
Anti-CT813+ND+
Anti-CT850+ND+
  • a Mouse antibodies raised with GST fusion proteins.

  • b RFP and GST were tagged to the N termini of chlamydial proteins. IFA, immunofluorescence assay; WB, Western blot assay; +, positive reaction; −, negative reaction; ND, not detected.

  • c These five antibodies only reacted with the corresponding GST, but not RFP, fusion proteins, suggesting that these antibodies cannot recognize C. trachomatis-specific proteins.