Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype or phenotypeSource or reference
E. coli strains
    DH5FrecA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1Vicky Shingler
    S17-1λpir recA thi pro hsdRM+ Smr <RP4-2-Tc-Mu-Ku-Tn7> Tpr 75
    BL21(DE3) plysSFdcm lon ompT hsdS (rB mB) gal λ(DE3) [pLysS Cmr]Promega
Y. pseudotuberculosis a strains
    YPIII/pIB102 yadA::Tn5 Kmr (parent) 4
    YPIII07/pIB102 cpxA in-frame deletion of codons 41-449 Kmr 6
    YPIII07/pIB75 yscU in-frame deletion of codons 25-329 KmrThis study
    YPIII08/pIB102 cpxR in-frame deletion of codons 11-193 Kmr 6
    SF104/pYH7ΔyadA (in frame) inv::kan Kmr 30
    pLS13669-bp PCR fragment of the allele encoding YscUΔ25-329 on pDM4; Cmr 43
    pCR4-TOPOTA cloning vector; Kmr CbrInvitrogen
    pMMB208Expression vector; Cmr 50
    pKEC021∼700-bp XbaI/KpnI PCR fragment of cpxR in pMMB208; Cmr 6
    pJF015∼720-bp XbaI/KpnI PCR fragment of cpxR encoding D51A mutation in pMMB208; Cmr 6
    pET22b(+)Expression vector; CbrNovagen
    pMF5811,381-bp NdeI/EagI PCR fragment of cpxA in pET22b(+); Cbr 6
    pKEC017∼700-bp NdeI/XhoI PCR fragment of cpxR in pET22b(+) that creates a C-terminal His6 fusion; CbrThis study
    pGN37pBAD18 rovA+; Cbr 31
    pIRR1∼4.4-kb BamHI fragment of inv in pACYC184; Cmr 70
  • a To the best of our knowledge, the Y. pseudotuberculosis strains are all isogenic according to information from literature resources (30, 36, 70).