Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype or phenotypeSource or reference
E. coli strains
    DH5F recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1Vicky Shingler
    S17-1λpirrecA thi pro hsdRM+ Smr <RP4-2-Tc-Mu-Ku-Tn7> Tpr75
    BL21(DE3) plysSF dcm lon ompT hsdS (rB mB) gal λ(DE3) [pLysS Cmr]Promega
Y. pseudotuberculosisa strains
    YPIII/pIB102yadA::Tn5 Kmr (parent)4
    YPIII07/pIB102cpxA in-frame deletion of codons 41-449 Kmr6
    YPIII07/pIB75yscU in-frame deletion of codons 25-329 KmrThis study
    YPIII08/pIB102cpxR in-frame deletion of codons 11-193 Kmr6
    SF104/pYH7ΔyadA (in frame) inv::kan Kmr30
    pLS13669-bp PCR fragment of the allele encoding YscUΔ25-329 on pDM4; Cmr43
    pCR4-TOPOTA cloning vector; Kmr CbrInvitrogen
    pMMB208Expression vector; Cmr50
    pKEC021∼700-bp XbaI/KpnI PCR fragment of cpxR in pMMB208; Cmr6
    pJF015∼720-bp XbaI/KpnI PCR fragment of cpxR encoding D51A mutation in pMMB208; Cmr6
    pET22b(+)Expression vector; CbrNovagen
    pMF5811,381-bp NdeI/EagI PCR fragment of cpxA in pET22b(+); Cbr6
    pKEC017∼700-bp NdeI/XhoI PCR fragment of cpxR in pET22b(+) that creates a C-terminal His6 fusion; CbrThis study
    pGN37pBAD18 rovA+; Cbr31
    pIRR1∼4.4-kb BamHI fragment of inv in pACYC184; Cmr70
  • a To the best of our knowledge, the Y. pseudotuberculosis strains are all isogenic according to information from literature resources (30, 36, 70).