TABLE 2.

Oligonucleotides used in this study

Application and specific geneaNameSequenceb
RT-PCR
    rpoA (330 bp)prpoAa5′-GTT CGA CGC ACG CCA AGG TGA-3′
prpoAb5′-ACG TCC TGC GGC TTG ACG AT-3′
    cpxR (390 bp)pcpxRfor5′-GTG AAC TGA CGT CGC TGT TGA-3′
pcpxRrev5′-TTG CAG GCA ATC AAC TTC CAG-3′
    cpxA (295 bp)pcpxAkpn5′-ACG TAC GGT ACC ATC AGT GCA CCG CTG-3′
pcpxAsac5′-ACG TAC GAG CTC TTC ACC ATG ACG GCG-3′
    cpxP (220 bp)pcpxPfor5′-GTT ATG GCG TCA ATG TTC GTT C-3′
pcpxPrev5′-CAA GAT CAA GGC GCG GCT GAC-3′
    ppiA (210 bp)pppiAfor5′-CCG GGA ATA TTG AGC TGG AG-3′
pppiArev5′-CGC AGG CCA TTA TCT GCT TC-3′
    degP (410 bp)pdegPkpn5′-ACG TAC GGT ACC ATC TGA CTG CGA TTA-3′
pdegPsac5′-ACG TAC GAG CTC CAA CCT TCA TGG CTT-3′
    ail (240 bp)pail2867for5′-ATT GCA TGT TTA TCA ATT GCG-3′
pail2867rev5′-CCA TCA ATA AGT TTG AAT CCG-3′
    ail-like (280 bp)pail2113for5′-CAC GGT ATC TTT CGG TTA CGC-3′
pail2113rev5′-TGT ACT CTT ACC TTG AGT TGC-3′
    ail2 (200 bp)pail1731for5′-CAG GGA GAT GTA AGA CTC GGT-3′
pail1731rev5′-GCG AAT AAT AAT CAA AGG ATG-3′
    ompX (235 bp)pompXfor5′-TTG CAT GTC TTT CAG CGG TAG-3′
pompXrev5′-TTG TAA ACC GCT TCG TCA CCG-3′
    psaA (265 bp)ppsaAfor5′-ATC GCT GCT TGT GGT ATG GC-3′
ppsaArev5′-ACC AAC ATA GTC ACC ATC GG-3′
    inv (235 bp)pinvfor5′-CCA GCC TTA TTC TGT CTC TTC-3′
pinvrev5′-CCG CAT CGC CCA CCA TTG AG-3′
    rovA (230 bp)provAfor5′-GGC GCG CAT TAA TTG ACC ATC-3′
provArev5′-AAT TCT CTT CGC ACG ACG ATC-3′
    hns (345 bp)phnskpn5′-ACG TAC GGT ACC TTG CTC TTC AAT GGC-3′
phnssac5′-ACG TAC GAG CTC TTA ACA ACA TCC GTA-3′
Protein expression
    cpxR::his(6) (700 bp)pcpxR-Nde(ET)5′-CAT ATG CAT AAA ATC CTA TTA GTT GAT G-3′
pcpxR-Xho(HisET)5′-CTC GAG TGT TTC TGA TAC CAT CA-3′
Mobility shift assay
    cpxR/cpxP (245 bp)pcpxRb5′-GTC ATC ATC AAC TAA TAG GA-3′
pcpxPb5′-AAC GAA CAT TGA CGC CAT AAC-3′
    cpxR (internal, 390 bp)pcpxRfor5′-GTG AAC TGA CGT CGC TGT TGA-3′
pcpxRrev5′-TTG CAG GCA ATC AAC TTC CAG-3′
    degP (245 bp)pdegPfulla5′-ACG CTC GAG CTG CGG AAT AGT ATG CA-3′
pdegPfullb5′-CAA TGC CAA TGC ACT TAA TAC-3′
    rovA (715 bp)provAfor15′-CCG ACG CTA AGT GTC AAT AAC-3′
provArev15′-GAA CTA ATC GTG CTA GAT CAG-3′
    inv (505 bp)pinvfor15′-TCA TCA AGG CAA CCA TCA GGA-3′
pinvrev15′-AGA AAC TCA CTG ATT GGC TGG-3′
  • a The number of base pairs in parentheses is the approximated size of the amplified PCR fragment.

  • b The NdeI and XhoI restriction endonuclease sites are in italics.