TABLE 3.

Changes in the expression of AI-2 uptake genes in E. coli O157:H7 biofilms after exposure to EPI and NE

GeneBiological process descriptionRelative change in expression (n-fold) determined by the indicated methoda
MicroarrayRT-PCR (EPI)
EPINE
lsrB AI-2 transporter −7.0 −5.3 −2.5
lsrG Antibiotic biosynthesis −3.2 −3.0
lsrD Transport −3.2 −1.4
lsrA Transport −9.8 −5.3 −4.8
lsrC Transport −4.3 −2.3 −2.4
lsrK Carbohydrate metabolism −2.8 −2.3
lsrR Regulation of transcription; DNA dependent −3.7 −3.7
  • a Values are based on an exposure of 7 h. Significant changes are in boldface.