Table 1.

Subcellular localization of CFP29 and PstS

Subcellular fractionaReactivitybwith protein (MAb):
CFP29 (HYB 71-2)PstS (HBT12)
Cell wall++
Membrane, aq.++++
Membrane, det.+++
  • a aq. and det., aqueous and detergent phases, respectively, obtained after Triton X-114 phase separation. The same quantity of total protein was applied for all samples, except for the aqueous and detergent phases, which in total represented the same protein content as the other fractions.

  • b Values from immunoblot analysis: −, no reactivity, +, weak reactivity, ++, intermediate reactivity, +++, strong reactivity.