Table 1.

Strains used in this study

Species and strainCharacteristicsSource or reference
B. pertussis
 18323 (ATCC 9797)Wild type, SmsATCC
 SK6vrg6::TnphoASmr; original mutant6
 NSK6vrg6::TnphoASmr; 18323 transductantThis work
 SC3bvgS-C3 SmsThis work
 D6Δvrg6 Sms; in-frame deletion invrg6This work
 DRΔbvgR Sms; in-frame deletion in bvgRThis work
 DS1bvgS::pGMT74 Sms;bvgS disruptionThis work
 SC3-SK6bvgS-C3vrg6::TnphoA Smr; SC3 transductantThis work
 DR-SK6ΔbvgR vrg6::TnphoA Smr; DR transductantThis work
 DS1-SK6bvgS::pGMT74vrg6::TnphoA SmrThis work
 BP121vrg6::TnphoA′-′tnpR-res-tet-res-′vrg6SmrThis work
 BP147vrg6::TnphoA′-′tnpR-res-tet-res-vrg6SmrThis work
 NBP121vrg6::TnphoA′-′tnpR-res-tet-res-′vrg6Smr; 18323 transductantThis work
 NBP147vrg6::TnphoA′-′tnpR-res-tet-res-vrg6Smr; 18323 transductantThis work
E. coli
 DH5αFhsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(argF-lac) U169φ80dlacZΔM15Bethesda Research Laboratories
 SM10RP4-2-TcMu Kmr28
 DM1187(pCLB1)Colicin B-expressing strainTheresa Finn