Table 1.

Effect of rLBP on LPS-induced TNF-α, IL-1β, and IP-10 genes in murine macrophages

TreatmentLevela of:
TNF-αIL-1βIP-10
2 ng of LPS per ml + 2% FBS100100100
2 ng of LPSb 172 ± 9401 ± 8344 ± 6
2 ng of LPS per ml + 0.1 ng of rLBP per mlb 151 ± 18327 ± 9959 ± 12
2 ng of LPS per ml + 1 ng of rLBP per mlb 135 ± 23232 ± 94122 ± 19c
2 ng of LPS per ml + 10 ng of rLBP per mlb 99 ± 5c 109 ± 18c 128 ± 13c
2 ng of LPS per ml + 100 ng of rLBP per mlb 94 ± 4c 96 ± 9c 110 ± 9c
  • a Arithmetic mean ± standard error of the mean (SEM) from six separate experiments for LPS alone and LPS with 100 ng of rLBP per ml, arithmetic mean ± SEM from three separate experiments for LPS with 1 or 10 ng of rLBP per ml and LPS with FBS, and arithmetic mean ± SEM from two separate experiments for LPS with 0.1 ng of rLBP per ml. All values are percentages.

  • b PhosphorImager data were normalized for the housekeeping gene β-actin before being expressed as a percentage of the LPS response in the presence of 2% FBS as described in Materials and Methods.

  • c Significantly different (P ≤ 0.05) from value for LPS alone under serum-free conditions, as determined by an unpaired Student’s t test.