Table 2.

Edman sequencing of mutacin 1140 derivatized with ethanethiol

CycleResidue predicted from DNA sequenceIdentified residue
1PhePhe
2LysLys
3SerS-ECa
4TrpTrp
5SerS-EC
6LeuLeu
7CysS-EC
8Thrβ-M-S-ECa
9ProPro
10GlyGly
11CysS-EC
12AlaAla
13ArgArg
14Thrβ-M-S-EC
15GlyGly
16SerS-EC
17PhePhe
18AsnAsn
19SerS-EC
20TyrTyr
21CysNDb
22CysND
  • a Thioethyl cysteine (S-EC) derived from ethanethiol treatment of lanthionine, 3-methyllanthionine, and 2,3-didehydroalanine; β-methylthioethyl cysteine (β-M-S-EC) derived from ethanethiol treatment of 3-methyllanthionine and 2,3-didehydrobutyrine according to the scheme of Meyer et al. (17).

  • b ND, not detected.