Table 3.

Mucosal antibody responses induced by rUre immunization

RouteaVaccinebChallengecMean level of IgAd (ng/ml) in:
Gastric secretionsSaliva
p.o.rUre + LT0.31798.56
+6.00e 832.12
r.rUre + LT40.89e 82.16
+72.40e 176.65
i.n.rUre + LT0.25439.23
+1.02e 1,258.72f
AllLT+0.120.10
  • a Weekly immunizations were given on days 0, 7, 14, and 21 by either the p.o. (50 μl of soluble antigen delivered by pipette into the buccal cavity), the i.n. (5 μl of soluble antigen delivered to each external naris of unanesthetized mice), or the rectal (r.) (25 μl of soluble antigen delivered with an olive-tipped catheter directly into the rectum) route. Sham-immunized control animals (n = 15) received LT alone by either the p.o., i.n., or rectal route, and results were pooled for this analysis (“All”).

  • b By the p.o. or rectal route, animals were administered 25-μg doses of rUre plus LT (5 μg p.o. and 25 μg rectally), and by the i.n. route, animals were administered 10-μg doses of rUre plus LT (5 μg) (n = 10/group).

  • c Animals were challenged once with 100 μl of a 108-CFU/ml suspension of streptomycin-resistant H. pylori X472AL.

  • d Gastric and salivary IgA antibody responses are presented as geometric means.

  • e Statistical significance (P< 0.005) was determined by the Wilcoxon rank sum test, with results for immunized animals being compared to those for sham-immunized controls.

  • f Statistical significance (P< 0.05) was determined by the Wilcoxon rank sum test, with results for challenged animals being compared to those for similarly treated unchallenged animals.