Table 1.

Vaccine-induced protection in the mouse model

Immunization groupLog10 mean viable bacteria in the lung ± SEaLog10 protectionMean DTH response (mm) ± SDb
PBS4.77 ± 0.0700 ± 0
BCG3.43 ± 0.22*1.340.4 ± 0.28
MPL4.88 ± 0.08−0.120 ± 0
MPL-CFP5.03 ± 0.21−0.270 ± 0
MPL–IL-124.81 ± 0.08−0.050 ± 0
MPL–CFP–IL-124.46 ± 0.110.310.025 ± 0
MPL–CFP–IL-24.58 ± 0.160.180 ± 0
MPL–CFP–IL-12–IL-23.86 ± 0.08*0.910 ± 0
  • a Protective efficacy in mice of a subunit vaccine containing MPL–CFP–IL-12–IL-2 4 weeks after an aerogenic infection with M. tuberculosis. Data are presented as log10 CFU ± standard error (SE), wheren = 4. Asterisks represent statistical significance (P < 0.05) based on the unpaired, two-tailed Studentt test.

  • b DTH response measured 48 h after injection of PPD into the left footpads of vaccinated mice 2 days before infection with M. tuberculosis. Data are presented as the difference in footpad thickness (left footpad thickness [PPD] minus right footpad thickness [saline]).