TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristics or genotypeaSource
Strains
    E. coli
        DH5αendA1 hsdR17 (rK mK) supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 deoR [φ 80dlacΔ(lacZΔ M15)]Invitrogen
    C. jejuni
        AS144C. jejuni NCTC 11168NCTC
        AS53C. jejuni TGH9011 (ATCC 43431)ATCC
        AS275C. jejuni 81-176C. L. Pickett
        AS217AS144 feoB::KmrThis study
        AS236AS53 feoB::KmrThis study
        AS237AS275 feoB::KmrThis study
Plasmids
    pILL600Kmr resistance geneA. Labigne
    pCAPCloning and suicidal vector; AmprRoche
    pAS43pCAP carrying feoBThis study
    pAS223pCAP carrying feoB::KmrThis study
  • a Ampr, ampicillin resistance gene.