TABLE 3.

Sequences for bla SS, bla SS-CT, ompA SS, and phoA SS and their fusions to pspA and pspC

Signal sequence (no. of amino acids)DNA sequence in expression plasmid or corresponding amino acid(s)aReference
bla SS (36)ATG AGT ATT CAA CAT TTC CGT GTC GCC CTT ATT CCC TTT TTT GCG37
M S I Q H F R V A L I P F F A
GCA TTT TGC CTT CCT GTT TTT GCT CAC CCA GAA ACG CTG GTG AAA
A F C L P V F A H P E T L V K
GTA AAA GAT GCT GAA TTC (EcoRI [pspA or pspC])
V K D A ↑ E F
bla SS+CT (59)ATG AGT ATT CAA CAT TTC CGT GTC GCC CTT ATT CCC TTT TTT GCG GCA TTT TGC45
M S I Q H F R V A L I P F F A A F C
CTT CCT GTT TTT GCT CAC CCA GAA ACG CTG GTG AAA GTA AAA GAT GCT GAA
L P V F A H P E T L V K V K D A ↑ E
TTC (EcoRI [pspA or pspC])
F
(PstI) CTG CAG GCA ACT ATG GAT GAA CGA AAT AGA CAG ATC GCT GAG ATA GGT GCC TCA
L R A T M D E R N R Q I A E I G A S
CTG ATT AAG CAT TGG TAA AAG CTT (HindIII)
L I K H W
ompA SS (24)ATG AAA AAG ACA GCT ATC GCG ATT GCA GTG GCA CTG GCT GGT TTC GCT47
M K K T A I A I A V A L A G F A
ACC GTA GCG CAG GCC GCG GAA TTC (EcoRI [pspA or pspC])
T V A Q A A ↑ E F
phoA SS (28)ATG AAA CAA AGC ACT ATT GCA CTG GCA CTG CTG CCG CTG CTG TTT40
M K Q S T I A L A L L P L L F
ACC CCT GTG ACC AAA GCC CGT ACC CCA GAA ATG AAC CCG GGG ATC C (BamH [pspA or pspC])
T P V T K A ↑ R T P E M N P G I
  • a The predicted cleavage site of each signal sequence is indicated by an arrowhead. Restriction sites (underlined) are defined in parentheses.