TABLE 2.

Plasmids used in this study

PlasmidRelevant characteristic(s)Derivation or reference
pIN-III-ompASecretion vector with ompA signal sequence50
TOPO-TACommercial vector for TA cloningInvitrogen
pYA3342Asd+ expression vector Ptrc promoter pBRori39
pYA3493Asd+ vector bla SS-based secretion periplasmic plasmid pBRori39
pYA3620Asd+ vector bla SS+CT-based secretion periplasmic plasmid pBRori14
pYA37441.2-kb DNA encoding the α-helical region of PspC (aa 4-404) in pYA4106This study
pYA38020.8-kb DNA encoding the α-helical region of PspA (aa 3-285) in pYA3620This study
pYA40281.2-kb DNA encoding the α-helical region of PspC (aa 4-404) in pYA3493This study
pYA40880.8-kb DNA encoding the α-helical region of PspA (aa 3-285) in pYA3493This study
pYA4096His-tagged PspC (aa 4-404) in pET28aThis study
pYA40981.5-kb DNA encoding the codon-optimized α-helical and proline-rich regions of PspC (aa 4-477) in TOPO vectorThis study
pYA4102Asd+ vector ompA SS-based plasmid pBRoriThis study
pYA4106Asd+ vector phoA SS-based plasmid pBRoriThis study
pYA42021.2-kb DNA encoding the α-helical region of PspC (aa 4-404) in pYA4102This study
pYA42660.8-kb DNA encoding the α-helical region of PspA (aa 3-285) in pYA4102This study
pYA42670.8-kb DNA encoding the α-helical region of PspA (aa 3-285) in pYA4106This study
pYA42691.2-kb DNA encoding the α-helical region of PspC (aa 4-404) in pYA3620This study
pYA42701.2-kb DNA encoding the α-helical region of PspC (aa 4-404) in pYA3342This study
UAB055α-helical region of PspA (aa 1-302) in pET20b3