Primers used in this study
| Primer purpose and name | Gene | Sequence |
|---|---|---|
| Cloning ompA SS and phoA SS | ||
| P1 | ompA SS | 5′-TCATGAAAAAGACAGCTATCGCGATTGCA-3′ |
| P2 | ompA SS | 5′-ACGGAATTCAGCGGCCTGCGCTACGGTAGCGAAACC-3′ |
| P3 | phoA SS | 5′-TCATGAAACAAAGCACTATTGCACTGGCA-3′ |
| P4 | phoA SS | 5′-ACGGATCCCCGGGTTCATTTCTGGGGTACGGGC-3′ |
| Cloning pspA | ||
| P5 | pspA | 5′-GGAATTCTCTCCCGTAGCCAGTCAGTCT-3′ |
| P6 | pspA | 5′-TTCAAGCTTATTATGCTTTCTTAAGGTCAGCTT-3′ |
| P7 | pspA | 5′-TTCCTGCAGATTATGCTTTCTTAAGGTCAGCTT-3′ |
| P8 | pspA | 5′-CATGGATCCGTTCTCCCGTAGCCAGTCAGTCT-3′ |
| Cloning pspC | ||
| P9 | pspC | 5′-ACGAATTCGAAGGCCTGCCAAGTACCACTTCTTC-3′ |
| P10 | pspC | 5′-GCTGGTCGACCTATTATTTTTCTTTAACTTTATC-3′ |
| P11 | pspC | 5′-GCTCTGCAGTTTTTCTTTAACTTTATC-3′ |
| P12 | pspC | 5′-ATACCCGGGGGAAGGCCTGCCAAGTACCAC-3′ |
| P13 | pspC | 5′-GCTCTGCAGCTATTATTTTTTTCTTTAACTTTATC-3′ |
| P14 | pspC | 5′-GATGAATTCGAAGGCCTGCCAAGTACCACT-3′ |
| P15 | pspC | 5′-CGAGGATCCATTATTTTTCTTTAACTTTATCTTC-3′ |
| P16 | pspC | 5′-ATGCCATGGAAGGCCTGCCAAGTACCACTTCTTC-3′ |