TABLE 1.

Bacterial strains, plasmids, and primers used in this study

Strain, plasmid, or primerDescriptionSource or reference
E. coli strains
    TOP10High-efficiency transformation strain; ZeosInvitrogen
    SM10Mobilizing strain; Kmr Pms Zeos34
B. pseudomallei strains
    DD5031026b derivative; Δ(amrR-oprA) rpsL; Pmr Zeos26
    ZP1220DD503 derivative; bsaZ::pZPbsaZ; Pmr Zeor KmsThis study
    ΔsctUBp3DD503 derivative; ΔbsaZ mutant; Kms44
    ΔsctUBp1,2,3DD503 derivative; T3SS-1, -2, -3 mutant; Kms44
Plasmids
    pEM7/ZeoSh ble cassette vector; ZeorInvitrogen
    pGSV3Mobilizable suicide vector; Gmr14
    pZSVMobilizable suicide vector; pGSV3 derivative; ZeorThis study
    pZPbsaZpZSV containing a 486-bp PCR fragment internal to bsaZ; ZeorThis study
Primers
    SV15′-AAATAGCGCCGAGCTTTGTC-3′This study
    BPbsaZ-F15′-GTACGCGAATTCCTCGTCGATCTGACGCGCATCGCG-3′aThis study
    BPbsaZ-R15′-GTACGCGCTAGCGTGGTACAGGAAGTATTCGACGGC-3′aThis study
    BPbsaZ-MF5′-CATCGTCGCGCTCATCGTGATCGC-3′This study
    MB1ori-P15′-GAAGATCCTTTGATCTTTTCTACGG-3′4
  • a Underlining indicates the EcoRI and NheI restriction sites in the linker regions.