TABLE 1.

Bacterial strains and plasmid constructs used in this study

Strain or plasmidRelevant characteristicsReference or source
Strains
    B. abortus
        2308 NalrVirulent WT strain, biotype 1, LPS-S, spontaneous Nalr mutant39
        bvrS mutant strain 2.132308 Nalr bvrS::Tn542
        bvrR mutant strain 65.212308 Nalr bvrR::Tn542
        bvrR+ strain 65.21p65.21 carrying plasmid pBBR2.1342
        omp3a mutant 2308Nalr omp3a::KmThis work
        omp3b mutant 2308Nalr omp3b::KmThis work
    E. coli
        SM10 (λ pir)thi-1 thr leu tonA lacY supE recA::RP4-2-Tet::Mu Kmr; λ pir40
        XL1-BlueTetr supE44 hsdR17 recA1 endA1 gyrA96 thi relA1 lac F′ [proAB+ lacIq lacZΔM15 Tn10 (Tetr)]38
        TOP 10 F′F′ [lacIq Tn10 (Tetr)] mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (Strr) endA1 nupGInvitrogen
Plasmids
    pCR2.1High-copy-number cloning vector; Ampr KmrInvitrogen
    pUC4KPlasmid containing kanamycin resistance cassetteStratagene
    pSK-oriTpBluescript II SK(-) oriT RK2 Ampr43
    pBBR1MCS-4Intermediate-copy-number cloning vector; Ampr25
    pAC2553pUC19 containing Δomp3a::KmA. Cloeckaert
    pSK3aKmpSK-oriT containing Δomp3a::Km (1.8 kb)This work
    pTA3apCR2.1 containing an internal fragment of B. abortus 2308 omp3a amplified by PCR (0.55 kb)This work
    pTA3bpCR2.1 containing complete B. abortus omp3b gene amplified by PCR (1.1 kb)This work
    pTA3bpCR2.1 containing deleted omp3b gene (0.6 kb)This work
    pSK3b-KmpSK-oriT containing Δomp3b::Km (1.9 kb)This work
    pBBR3bpBBR1MCS-4 containing B. abortus omp3b gene (1.1 kb)This work