Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionReference or source
M. catarrhalis
 035EWild-type isolate from middle ear fluid 27
 035E.1Isogenic mutant of 035E with a kan cartridge in the uspA1 structural gene 1
 035E.2Isogenic mutant of 035E with a kan cartridge in the uspA2 structural gene 1
 035E.12Isogenic mutant of 035E with a kancartridge in the uspA2 structural gene and a catcartridge in the uspA1 structural geneThis study
 P-44Wild-type isolate that exhibits rapid hemagglutination 52
 P-48Wild-type isolate that exhibits slow hemagglutination 52
Escherichia coli DH5αHost for cloning experimentsStratagene
Plasmids
 pBluescript II SK+Cloning vector; Ampr Stratagene
 pUSPA1pBluescript II SK+ with a 2.7-kb insert containing most of the uspA1 gene of M. catarrhalis035E 1
 pUSPA1CATpUSPA1 with acat cartridge replacing the 0.6-kb BglII fragment of the uspA1 geneThis study