Table 1.

Bacterial strains and plasmids

Strain or plasmidDescriptionSource or reference
Bordetellae
B. bronchiseptica5376Wild type 37
B. bronchisepticaCBF1Spontaneous Rifr mutant of 5376This work
B. bronchiseptica CBF2As CBF1 butxxx::Tn5 lux This work
B. bronchiseptica CBF3As CBF1 butxxx::Tn5 lux This work
B. avium 4148Wild type 39
B. aviumCAF1Spontaneous Rifr mutant of 4148This work
B. parapertussis 10520Wild typeNCTCa
B. parapertussisCPF1Spontaneous Rifr mutant of 10520This work
E. coli strains
 CC118λpir Δ(ara-leu) araD ΔlacZX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1pir) 23
 SM10λpir Kanr; thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu (λpir) 23
 DH5α supE44 ΔlacU (φ80lacZ M15)hsdR17 recA1 endA1 gyrA96 thi-1 relA1 22
Plasmids
 pT7-3Ampr; contains T7 promoter, φ10, and the luxCDABE genes from P. luminescens 46
 pUTmini-Tn5kmAmpr; Tn5-based delivery plasmid with Kanr 10
 pUTmini-Tn5km2Ampr; Tn5-based delivery plasmid with Kanr 10
 pUTmini-Tn5kmlux As pUTmini-Tn5km but carries the luxCDABE genes fromP. luminescens This work
 pUTmini-Tn5km2lux As pUTmini-Tn5km2 but carries the luxCDABE genes from P. luminescens G. Stewart, University of Nottingham, Nottingham, United Kingdom
  • a NCTC, National Collection of Type Cultures (Central Public Health Laboratory, London, England).