Table 2.

Chromosomal DNA from different strains of S. pyogenes used as targets in PCR amplifications with oligonucleotides derived from the ORF sequence, the emm oremm-like genes, or scpA (see Fig. 1B)

StrainaOFSize of PCR product (bp) generated with primer pair indicated
3244-3267A16-3267A16-4419ES1-32443244-44193266-3267
AP11,0001,5001,6002,800350
Umeå1,0001,5001,6002,800350
AP121,0001,5002,800350
AP551,0001,5002,800350
AP61,000500350
AP231,000500350
AP531,000500350
AP5500
AP57500
AP2+500350
AP4+500350
AP25+500350
AP58+500350
  • a The number denotes the M protein serotype. Umeå refers to eight strains of the M1 serotype isolated from patients with septicemia (16).