TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristicsaSource or reference
Plasmids
    pSM20Knr gfp+27
    pJIR750Cmr oriCP oriEC9
    pALK1pSM20 Ω (BamHI-SpeI:pJIR750, 0.9 kb) Δgfp (Cmr Knr)Recombinant
    pALK2pALK1 Ω (1.8-kb upstream plc fragment, catP, 2.1-kb downstream plc fragment) (Cmr Knr)Recombinant
Strains
    JIR325Strain 13 Rifr Nalr25
    JIR4107JIR325 plc::erm(B)4
    ATCC 13124Type A (plc+)
    CN 1884Type B (plc+ cpb+ etx+)R. G. Wilkinson (Univ. Melbourne)
    CN 2109Type C (plc+ cpb+)R. G. Wilkinson (Univ. Melbourne)
    CN 462Type D (plc+ etx+)R. G. Wilkinson (Univ. Melbourne)
    ATCC 27324Type E (plc+ cpi+ cpe+ cpb2+)
    NE18-M1EHE-NE18 Δplc::catPThis work
    NE18-M4EHE-NE18 Δplc::catPThis work
    NAG-NE1Isolated from gut contents of chicken with NE, flock 137
    EHE-NE3Isolated from liver of chicken with NE, flock 237
    EHE-NE4Isolated from liver of chicken with NE, flock 237
    EHE-NE5Isolated from kidney of chicken with NE, flock 237
    EHE-NE7Isolated from gut wall of chicken with NE, flock 237
    EHE-NE9Isolated from gut wall of chicken with NE, flock 237
    EHE-NE13Isolated from gut wall of chicken with NE, flock 237
    EHE-NE14Isolated from gut contents of chicken with NE, flock 237
    EUR-NE15Isolated from gut contents of chicken with NE, flock 337
    EHE-NE16Isolated from gut contents of chicken with NE, flock 437
    EHE-NE17Isolated from gut contents of chicken with NE, flock 437
    EHE-NE18Isolated from gut contents of chicken with NE, flock 437
    EHE-NE20Isolated from gut contents of chicken with NE, flock 5This work
    EHE-NE21Isolated from gut contents of chicken with NE, flock 5This work
    EHE-NE22Isolated from gut contents of chicken with NE, flock 5This work
    NAG-NE23Isolated from gut wall of chicken with NE, flock 637
    NAG-NE24Isolated from gut wall of chicken with NE, flock 637
    NAG-NE25Isolated from gut wall of chicken with NE, flock 637
  • a Cmr, chloramphenicol resistant; Knr, kanamycin resistant; Nalr, nalidixic acid resistant; Rifr, rifampin resistant.