TABLE 2.

Alpha-toxin and θ-toxin production and PCR-based toxin typing of C. perfringens isolates derived from chickens diagnosed with NE

Strain no.Toxin typeAlpha-toxinb (U mg−1 protein)θ-toxinb (log2 [titer])
NAG-NE1A(2.3 ± 1.0) × 10−36.3 ± 0.2
EHE-NE3A(1.9 ± 0.6) × 10−35.2 ± 1.1
EHE-NE4A(2.0 ± 0.7) × 10−35.8 ± 0.1
EHE-NE5A(13.3 ± 3.4) × 10−36.6 ± 0.1
EHE-NE7A(4.3 ± 3.8) × 10−36.6 ± 0.3
EHE-NE9A(8.3 ± 1.1) × 10−36.4 ± 0.1
EHE-NE13A(4.0 ± 0.8) × 10−35.7 ± 0.1
EHE-NE14A(4.0 ± 0.9) × 10−34.9 ± 0.1
EUR-NE15A(2.1 ± 0.2) × 10−34.4 ± 0.4
EHE-NE16A(5.2 ± 2.0) × 10−36.0 ± 0.2
EHE-NE17A(4.8 ± 0.8) × 10−37.9 ± 0.4
EHE-NE18A(1.9 ± 0.3) × 10−37.5 ± 0.1
EHE-NE20A(1.1 ± 0.3) × 10−34.7 ± 0.1
EHE-NE21A(2.7 ± 0.1) × 10−35.4 ± 0.3
EHE-NE22A(2.3 ± 0.6) × 10−36.0 ± 0.2
NAG-NE23A (cpb2+)(2.6 ± 1.4) × 10−36.2 ± 0.4
NAG-NE24A (cpb2+)(2.0 ± 0.5) × 10−36.0 ± 0.2
NAG-NE25A(3.6 ± 1.2) × 10−35.6 ± 0.5
JIR325aA(16.6 ± 3.3) × 10−37.5 ± 0.3
  • a A derivative of strain 13, a human gas gangrene isolate, used as a control.

  • b Results represent averages of duplicate assays carried out on preparations from at least three separate cultures of each strain, ± standard deviations.