TABLE 1.

Host cell membrane and nuclear proteins, identified by mass spectrometry, that are susceptible to proteolysis by Sat

Band submitted (molecular mass [kDa])DatabaseMass match (%)ToleranceMolecular mass (kDa)Identified protein(s)
Bladder membrane (>250)Swiss-Prot830.789372Bullous pimphigoid antigen 1 (BPAG1); contains 30 beta-spectrin repeats (4)
830.952288Nonerythroid spectrin beta IV
NCBI830.789372Bullous pimphigoid antigen 1 (BPAG1); contains 30 beta-spectrin repeats (4)
830.952289Nonerythroid spectrin beta IV
Kidney membrane
    1 (>250)Swiss-Prot1001.07416Spectrin, nonerythroid beta-chain 4
800.875288Spectrin, nonerythroid beta-chain 3
NCBI1001.45372Bullous pimphigoid antigen 1 (BPAG1); contains 30 beta-spectrin repeats (4)
1001.07416Spectrin, nonerythroid beta-chain 5
    2 (100-150)Swiss-Prot661.78128Leukocyte-function associated molecule 1
NCBI661.78128Leukocyte-function associated molecule 1
Bladder nucleus
    1 (>250)Swiss-Prot770.944409Abnormal spindle-like microencephaly-associated protein = MAP
771.37453AKAP450 (centrosome- and Golgi complex-localized PKN-associated protein) = MAP
NCBI771.37452AKAP450 (centrosome- and Golgi complex-localized PKN-associated protein) = MAP
    2 (100-150)Swiss-Prot661.33192LIM domain-only protein 7
NCBI661.33192LIM domain-only protein 7
    3 (100-150)Swiss-Prot661.20199Signal-induced proliferation-associated 1-like protein 1
NCBI661.20199Signal-induced proliferation-associated 1-like protein 1
Kidney nucleus
    1 (>250)Swiss-Prot800.976306MAP 1A (proliferation-related protein P80)
NCBI800.976306MAP 1A (proliferation-related protein P80)
800.976202MAP 1A (proliferation-related protein P80)
    2 (100-150)Swiss-Prot850.818113PARP
NCBI850.818113PARP