TABLE 1.

Bacterial strains and plasmids used in this study

Bacterial strain/plasmidRelevant phenotype, description, and/or selective markeraSource or reference
Strains
    Escherichia coli DH5αF′ φ80dlacZ ΔM15 Δ(lacZYA · argF)U169 deoR recA1 endA1 hsdR17(rK mK+) phoA supE44 λ thi-1 gyrA96 relA1Takara Bio, Otsu, Japan
    Porphyromonas gingivalis
        ATCC 33277Wild typeAmerican Type Culture Collection
        ATCC 33277 htpG strainErmrThis study
        ATCC 33277 wecA strainErmrThis study
        ATCC 33277 wbaP strainErmrThis study
        ATCC 33277 galE strainErmrThis study
        ATCC 33277 galE-c strainTetr ErmsThis study
        ATCC 33277 wzt strainErmrThis study
Plasmids
    pUC19Cloning vector; AmprTakara Bio, Otsu, Japan
    pBR322Cloning vector; AmprTakara Bio, Otsu, Japan
    pHS19Contains the ermF-ermAM cassette between EcoRI and BamHI sites of pBR322 without bla; Ermr10
    pBR322-ermContains the ermF-ermAM cassette between EcoRI and BamHI sites of pBR322; Ampr ErmrThis study
    pRN2Contains PG0045 between KpnI and AvaI sites of pUC19; AmprThis study
    pRN2-ermContains the ermF-ermAM cassette in PmaCI site within PG0045 of pRN2; Ampr ErmrThis study
    pRN3Contains PG0106 between XbaI and BamHI sites of pUC19; AmprThis study
    pRN3-ermContains the ermF-ermAM cassette in BglII site within PG0106 of pRN3; Ampr ErmrThis study
    pRN4Contains PG1964 between EcoRI and XbaI sites of pUC19; AmprThis study
    pRN4-ermContains the ermF-ermAM cassette in Bsp1407I site within PG1964 of pRN4; Ampr ErmrThis study
    pRN6Contains PG0347 between EcoRI and BamHI sites of pUC19; AmprThis study
    pRN6-ermContains the ermF-ermAM cassette in BseRI site within PG0347 of pRN6; Ampr ErmrThis study
    pRN7Contains PG1225 between XbaI and BamHI sites of pUC19; AmprThis study
    pRN7-ermContains the ermF-ermAM cassette in ApaI site within PG1225 of pRN7; Ampr ErmrThis study
    pKD375Contains tetQ cassette in pUC19; Ampr Tetr25
    pUC19QContains tetQ cassette between SmaI and BamHI sites in pUC19; Ampr TetrThis study
    pQGContains an entire region of galE and the 0.3-kb downstream region between BamHI and XbaI sites of pUC19Q; Ampr TetrThis study
    pGQGContains a region of 0.8 kb upstream of galE between KpnI and SmaI sites of pQG; Ampr TetrThis study
  • a Ampr, ampicillin resistant; Ermr, erythromycin resistant; Erms, erythromycin sensitive; Tetr, tetracycline resistant.