TABLE 2.

Increasing the dose of IMX108 increases the antibody responsea

AntigenDose (μg)Dose (nmol)IFA titerELISA titer
IMX10850.241/10,2401/102,400
100.481/10,2401/102,400
200.951/10,2401/102,400
401.91/20,4801/204,800
803.81/20,4801/204,800
1406.71/40,9601/409,600
2009.51/40,9601/409,600
IMX183231.91/80<100
  • a Groups of three BALB/c mice were immunized subcutaneously three times at 4-week intervals with different amounts of the IMX108 protein (MSP119-murine C4bp) without additional adjuvant. Control mice received IMX183 (MSP119 alone). Antibody titers were determined by IFA or by ELISA using pooled serum samples collected from each group 3 weeks after the third immunization. The IFA provided a measure of the amount of antibody binding to native MSP1 on the parasite, whereas the ELISA provided a measure of the amount of antibody binding to the recombinant MSP119.