TABLE 4.

Mean concentrations of salmochelins detected in culture supernatants

Strain (genotype)Concn (μM) of salmochelina
Monoglucosylated moleculesDiglucosylated moleculesTriglucosylated molecules
MGELinear MGES1SXDGE (S4)Linear DGE (S2)S5TGELinear TGE
χ71221.7 ± 0.281.1 ± 0.115.1 ± 0.129.8 ± 0.330.31 ± 0.031.1 ± 0.020.45 ± 0.02NDND
χ7304bNDNDNDNDNDNDNDNDND
χ7304(pYA3661 iroBCDEN)b0.22 ± 0.020.14 ± 0.039.2 ± 0.9431.0 ± 4.40.06 ± 0.0042.9 ± 0.071.7 ± 0.18NDND
χ7304(pIJ34 iroBCDE)c0.09 ± 0.010.07 ± 0.018.6 ± 1.126.1 ± 3.90.04 ± 0.0058.8 ± 0.981.5 ± 0.23ND0.06 ± 0.01
χ7304(pIJ137 iroBCDN)c0.17 ± 0.0010.22 ± 0.019.9 ± 0.5520.4 ± 1.10.42 ± 0.0222.7 ± 1.11.5 ± 0.050.04 ± 0.0010.19 ± 0.01
χ7304(pIJ136 iroBCEN)c0.09 ± 0.010.06 ± 0.014.0 ± 0.3319.6 ± 1.80.23 ± 0.025.6 ± 0.941.7 ± 0.09ND0.04 ± 0.004
χ7304(pIJ53 iroB)cNDND0.04 ± 0.001NDNDNDNDNDND
χ7304(pIJ121 iroBC)c0.09 ± 0.01ND1.3 ± 0.023.2 ± 0.260.52 ± 0.025.4 ± 0.120.43 ± 0.020.25 ± 0.010.13 ± 0.01
χ7304(pIJ135 iroBCN)c0.13 ± 0.010.04 ± 0.0021.1 ± 0.043.5 ± 0.400.46 ± 0.015.1 ± 0.030.46 ± 0.040.29 ± 0.010.15 ± 0.01
χ7304(pIJ37 iroBDEN)cNDND1.3 ± 0.131.7 ± 3.5ND0.71 ± 0.070.05 ± 0.005NDND
  • a Values represent means ±standard errors of the means of at least three independently grown cultures. Bold values indicate significant differences (P < 0.05) using a two-tailed unpaired t test. ND, molecule not detected in samples.

  • b Statistical differences were determined by using χ7122 as the reference strain.

  • c Statistical differences were determined by using χ7304(pYA3661 iroBCDEN) as the reference.