TABLE 1.

Recombinant proteins used in this studya

ProteinAntigenOligomerization domainElution vol (ml)
IMX183MSP119None
IMX269MSP119None
IMX108MSP119Murine C4bp163
IMX180MSP119Human C4bp164
IMX272MSP119Archaeal Sm152
IMX262MSP119Chicken2 C4bp165
IMX314MSP119Chicken1 C4bp
IMX317MSP119Chicken2 C4bpΔ7161
IMX313MSP119Hybrid C4bpΔ7162
IMX63DsbANone
IMX253DsbAMurine C4bp
IMX259IF tagChicken2 C4bp
IMX117IF tagHuman C4bp
IMX273IF tagArchaeal Sm
IMX315NoneChicken2 C4bp
  • a Recombinant proteins used in this study were all prepared from E. coli. The IF tag functions like a histidine tag and facilitates purification; however, the murine C4bp domain was not well expressed using this tag, and it was replaced by the DsbA protein.