TABLE 1.

Bacterial strains and plasmids used for this study

Bacterial strain or plasmidGenotype or phenotypeaSource or reference
Strains
    χ7122Avian pathogenic; O78:K80:H9; gyrA Nalr44
    χ7304ΔiroBCDEN::nptII ΔiucABCD iutA::xylE Kmr Nalr13
    DH5αF (Δ80d lacZΔM15) Δ(lacZYA-argF)U169 endA1 recA1 hsdR17(rK mK+) deoR thi-1 supE44 gyrA96 relA1Bethesda Research Laboratories
Plasmids
    pACYC184p15A replicon; Cmr Tcr6
    pBC SK (−)ColE1 origin; CmrStratagene
    pYA3661pACYC184::iroBCDEN; Cmr13
    pIJ33pACYC184::iroN; CmrThis study
    pIJ34pACYC184::iroBCDE; CmrThis study
    pIJ37pACYC184::iroBDEN; CmrThis study
    pIJ53pACYC184::iroB; CmrThis study
    pIJ121pACYC184::iroBC; CmrThis study
    pIJ135pACYC184::iroBCN; CmrThis study
    pIJ136pACYC184::iroBCEN; CmrThis study
    pIJ137pACYC184::iroBCDN; CmrThis study
  • a Cm, chloramphenicol; Km, kanamycin; Nal, nalidixic acid.