TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)Source or reference
Strains
    E. coli
        BL21(DE3)F ompT hsdSB(rB mB) gal dcm (DE3)Invitrogen
        DH10BF mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu)7697 galU galK λ rpsL nupG/pMON14272/pMON7124Invitrogen
    S. agalactiae
        515Wild typeD. Kasper, Harvard Medical School, Boston, MA
        515 (pAMp)Wild-type complemented with pAM401/gbs80P+TThis study
        515ΔsrtAΔsrtAThis study
        515ΔsrtA (pAMp)ΔsrtA complemented with pAM401/gbs80P+TThis study
        515ΔsrtA+ΔsrtA complemented with pAM401-srtAThis study
        Δ59Δgbs5929
        Δ67Δgbs6729
        Δ150Δgbs15029
        Δ1405ΔSAG140529
        Δ1406ΔSAG140629
Plasmids
    pJRS2336.0 kb; Emr; ColE1 ori; temperature-sensitive E. coli-streptococcal shuttle vector28
    pJRS233-ΔsrtApJRS233-derived containing overlapping flanking sequences of srtA geneThis study
    pAM401/gbs80P+T11.5 kb; Cmr; ColE1 ori; E. coli-streptococcal shuttle vector pAM401 containing promoter of gbs8029
    pAM401-srtApAM401/gbs80P+T-derived containing entire srtA coding sequenceThis study