TABLE 5.

Mean concentrations of enterobactin-related molecules detected in culture supernatants

Strain (genotype)Concn (μM) of enterobactin-related moleculea:
Enterobactin(DHBS)3(DHBS)2DHBS
χ712212.1 ± 3.425.3 ± 4.633.1 ± 4.711.6 ± 1.2
χ7304b62.9 ± 7.262.7 ± 6.286.6 ± 9.325.9 ± 3.9
χ7304(pYA3661 iroBCDEN)b1.5 ± 0.161.9 ± 0.259.6 ± 1.0127.1 ± 3.7
χ7304(pIJ34 iroBCDE)c0.9 ± 0.070.9 ± 0.1110.9 ± 1.920.8 ± 3.1
χ7304(pIJ137 iroBCDN)c3.8 ± 0.145.1 ± 0.2322.2 ± 0.913.9 ± 0.8
χ7304(pIJ136 iroBCEN)c0.6 ± 0.060.8 ± 0.128.6 ± 0.5715.0 ± 1.3
χ7304(pIJ53 iroB)c1.6 ± 0.350.6 ± 0.100.5 ± 0.01ND
χ7304(pIJ121 iroBC)c3.7 ± 0.202.9 ± 0.0910.7 ± 0.41.6 ± 0.10
χ7304(pIJ135 iroBCN)c4.6 ± 0.363.9 ± 0.2911.8 ± 0.52.6 ± 0.15
χ7304(pIJ37 iroBDEN)c2.7 ± 0.253.7 ± 0.3515.3 ± 1.58.2 ± 1.12
  • a Values represent means ± the standard errors of the means of at least three independently grown cultures. Bold values indicate significant differences (P < 0.05) using a two-tailed unpaired t test. ND, molecule not detected in samples.

  • b Statistical differences were determined by using χ7122 as the reference strain.

  • c Statistical differences were determined by using χ7304(pYA3661 iroBCDEN) as the reference strain.