TABLE 1.

Constructs and L. monocytogenes strains used for this study

LLO proteinaLLO plasmidbLLO plasmid L. monocytogenes strain in background:
Δhlyc (DP-L2161)ΔactA Δhly (DP-L4813)
Wild typeDP-E4817DP-L4818DP-L4896
K25RDP-E4861DP-L4928DP-L4882
K25GDP-E4874DP-L4929DP-L4899
K25VDP-E4875DP-L4930DP-L4900
K25LDP-E4876DP-L4931DP-L4901
K25FDP-E4995DP-L4997DP-L4996
K25ADP-E4880DP-L4933DP-L4905
K25TDP-E4879DP-L4934DP-L4903
S44SDP-E4878DP-L4966
K25R S44SDP-E4912DP-L4967
K25G S44SDP-E4913DP-L4968
K25V S44SDP-E4914DP-L4969
K25L S44SDP-E4915DP-L4970
K25F S44SDP-E4921DP-L4971
K25A S44SDP-E4961DP-L4978
K25T S44SDP-E4962DP-L4979
  • a Mutant proteins are identified by their mutations.

  • b DP-E numbers indicate pPL2-LLO constructs in E. coli.

  • c Reference 32.