TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsaReference or source
E. faecalis strains
    OG1RFLaboratory strain; Rifr Fusr 10
    CK111(pCF10)Conjugative donor construct; Rifs Fuss Sper 5
    TX5470 bps deletion mutant of OG1RF; OG1RF Δbpsb 13
    TX5536 srtA deletion mutant of OG1RF; OG1RF ΔsrtAThis study
    TX5537 srtA and bps double-deletion mutant of OG1RF; OG1RF ΔsrtA ΔbpsThis study
E. coli strains
    EC1000 E. coli host strain for routine cloning of RepA-dependent plasmids 6
    TX5497EC1000(pTEX5497); Chlr EryrThis study
Plasmids
    pCJK47Plasmid for markerless exchange; carries oriTpCF10, lacZ, and P-pheS*; Eryr 4
    pTEX5497Plasmid for srtA deletion with flanking regions of srtA cloned into pCJK47; EryrThis study
  • a Abbreviations: Chl, chloramphenicol; Ery, erythromycin; Fus, fusidic acid; Gen, gentamicin; Rif, rifampin; Tet, tetracycline; Spe, spectinomycin. A superscript s designates sensitivity, and a superscript r designates resistance.

  • b Previous studies referred to bps as srtC.