TABLE 1.

Primers used in this study

PrimerNucleotide sequenceaRelevant characteristic(s)
PorA5′Fwd5′-CCCAAGCTTGCCGTCTGAATACATCCCGTCATTCCTCA-3′HindIII, uptake sequence
PorA5′Rev5′-CGATGCTCGCGACTCCAGAGACCTCGTGCGGGCC-3′NruI
PorA3′Fwd5′-GGAAGATCTGATTAAATAGGCGAAAATACCAGCTACGA-3′BglII, stop codons
PorA3′Rev5′-GCCGAATTCTTCAGACGGCGCAGCAGGAATTTATCGG-3′EcoRI, uptake sequence
PorAlcOFwd5′-AAGCTCTGCAGGAGGTCTGCGCTTGAATTG-3′PstI
PorAlacORev5′-CTTAAGGCATATGGGCTTCCTTTTGTAA-3′NdeI
PPA15′-GCGGCCGTTGCCGATGTCAGCC-3′
PPA25′-GGCATAGCTGATGCGTGGAACTGC-3′
N01-full-NdeI5′-GGGAATTCCATATGAAAAAAGCACTTGCCACAC-3′NdeI
NdeI-NspA 35′-GGAATTCCATATGTCAGAATTTGACGCGCAC-3′NdeI
HSF 01-NdeI5′-GGAATTCCATATGATGAACAAAATATACCGC-3′NdeI
HSF 02-NheI5′-GTAGCTAGCTAGCTTACCACTGATAACCGAC-3′NdeI
ProD15-51X5′-GGGCGAATTCGCGGCCGCCGTCAACGGCACACCGTTG-3′EcoRI
ProD15-525′-GCTCTAGAGCGGAATGCGGTTTCAGACG-3′XbaI
TnRD15-KpnI/XbaI5′-CGCCGGTACCTCTAGAGCCGTCTGAACCACTCGTGGACAACCC-3′KpnI and XbaI, uptake sequence
TnR03Cam(KpnI)5′-CGCCGGTACCGCCGCTAACTATAACGGTC-3′KpnI
PorA-015′-CGCCGGTACCGAGGTCTGCGCTTGAATTGTG-3′KpnI
PorA025′-CGCCGGTACCTCTAGACATCGGGCAAACACCCG-3′KpnI
BAD165′-GGCCTAGCTAGCCGTCTGAAGCGATTAGAGTTTCAAAATTTATTC-3′NheI, uptake sequence
BAD175′-GGCCAAGCTTCAGACGGCGTTCGACCGAGTTTGAGCCTTTGC-3′HindIII, uptake sequence
BAD185′-TCCCCCGGGAAGATCTGGACGAAAAATCTCAAGAAACCG-3′XmaI and BglII
BAD195′-GGAAGATCTCCGCTCGAGCAAATTTACAAAAGGAAGCCGATATGCAACAGCAACATTTGTTCCG-3′BglII and XhoI
BAD215′-GGAAGATCTCCGCTCGAGACATCGGGCAAACACCCG-3′BglII and XhoI
BAD205′-TCCCCCGGGAGATCTCACTAGTATTACCCTGTTATCCC-3′XmaI, BglII, and SpeI
  • a Restriction sites are in bold type; uptake sequences or stop codons are underlined.