TABLE 2.

Quantitation of ChIP assay resultsa

DNA amplifiedbImmune/preimmune ratio (mean ± SEM) forc:P value (H37Rv vs BCG)d
H37RvBCG
serC-Rv08852.6 ± 0.62.8 ± 0.20.76
frdA 1.6 ± 0.14.5 ± 0.1 0.001
Rv0950c-sucC1.7 ± 0.14.1 ± 0.1 0.0009
Rv01452.3 ± 0.12.0 ± 0.70.65
PE152.3 ± 0.42.1 ± 0.20.65
Rv1158c-Rv11591.5 ± 0.21.9 ± 0.20.30
Rv3857c1.7 ± 0.12.1 ± 0.40.38
Rv0019c0.9 ± 0.11.1 ± 0-
  • a PCR bands were scanned and analyzed with ImageQuant software.

  • b DNA amplified from the intergenic regions of divergent genes or upstream of others. sigA was amplified from within its ORF.

  • c Ratio of bands amplified from immunoprecipitated immune serum to bands amplified from the preimmune serum control. The results are presented as means ± standard errors of the means of three biological repeats.

  • d P values from H37Rv versus BCG comparisons were compared using a two-tailed t test. Comparisons with P values of <0.05 are shown in bold. It was not possible to calculate a P value for Rv0019c (indicated by the dash), due to the 0 value for the standard error of the means.