Table 1.

Progressive vlsE sequence variation during the course of infection of C3H/HeN and CB-17 (SCID) mice with B. burgdorferi B31-5A3a

Time postinoculationNo. of variants/totalbAvg no. of nucleotide changes/variant (±SE)cAvg no. of predicted amino acid changes/variant (±SE)c
C3H/HeNSCIDC3H/HeNSCIDC3H/HeNSCID
4 days3/71/816.7 ± 3.51011.0 ± 2.48
7 days4/118.8 ± 0.56.0 ± 1.1
14 days9/94/634.7 ± 4.712.0 ± 2.919.3 ± 2.16.8 ± 2.3
21 days6/635.8 ± 2.221.7 ± 0.8
28 days19/198/841.5 ± 2.322.6 ± 4.824.6 ± 0.912.4 ± 2.9
7 mo7/743.1 ± 1.827.9 ± 1.6
12 mo8/839.6 ± 1.625.4 ± 1.7
  • a Combined results from Fig. 2, 3, and 4and additional experiments. Mice were inoculated with 105B31-5A3, and B. burgdorferi was cultured from skin biopsies at the indicated time points. Clones isolated by subsurface plating were used as a source of template DNA for PCR, and the products were subjected to sequence analysis. Changes relative to the parental clone B31-5A3 were calculated as described in the text. In this analysis, identical variant clones from the same initial culture were considered siblings and were counted as a single variant.

  • b B. burgdorferi clones containing DNA sequence changes in the vlsE cassette region over the total clones examined. Sibling variants were counted as a single clone.

  • c Average nucleotide or predicted amino acid sequence changes relative to B31-5A3 per unique variant ± standard error (SE).