Table 2.

Determination of macrophage cytotoxicity

StrainTime (h)Amt of IL-1β (pg/ml)% of CytotoxicityaCFUb
M9OT-W14444ND
23759015
M9OT-55101.3ND
201.56.4 × 103
Hly+ CDEC11152ND
225.6988
HlyCDEC101.4ND
20210 × 104
  • a Percentage of cytotoxicity refers to LDH released from HMDM calculated as a percentage of maximal release from uninfected cultures.

  • b CFU obtained after 30 min of infection followed by 50 min of incubation in gentamicin-containing medium. ND, not determined.