TABLE 2.

Trypan blue staining of bone marrow neutrophils to determine the percentage of neutrophils that were phagocytic and the percentage of phagocytic neutrophils that were producing ROSa

Influenza infection or LPS treatment% of neutrophils that were phagocytic after time (min):% of phagocytic neutrophils that were producing ROS after time (min):
060060
None38.5 ± 4.376.3 ± 5.541.1 ± 16.0*93.7 ± 3.4
LPS, 8 h36.7 ± 2.692.9 ± 5.564.4 ± 4.495.9 ± 1.8
Influenza, 3 days50.7 ± 4.378.5 ± 6.955.5 ± 13.994.7 ± 1.3
Influenza, 6 days46.9 ± 3.971.8 ± 8.4**23.6 ± 6.4*†88.3 ± 2.4
  • a Values represent means ± standard deviations (3 mice/group). Neutrophils were collected from mice and incubated with DiI-labeled S. pneumoniae for 0 or 60 min to measure phagocytosis and ROS generation. Statistical differences between groups were calculated using one-way analysis of variance, followed by the Bonferroni post test. Differences between groups with the same incubation time are indicated as follows: *, P < 0.05; **, P < 0.01 compared to neutrophils from LPS-challenged mice; †, P < 0.05 compared to neutrophils from mice infected with influenza for 3 days.