TABLE 2.

Infectivities of noninfectious and lp28-1+ attenuated mutantsa

Strain and inoculumNo. of tissue samples infected/no. testedNo. of mice infected/no. testedNo. of mice reactive/no. testedc
EarBladderHeartJointAll sitesb
5A18 NP1 (parental strain)
    1020/40/40/40/40/160/40/4
    1043/44/44/44/415/164/44/4
    1064/44/44/44/416/164/44/4
    Single mouse (104)1/11/11/11/14/41/11/1
        Total8/139/139/139/1335/529/139/13
MG001 (insertion site ND; no plasmids lost)
    1020/40/40/40/40/160/40/4
    1040/40/40/40/40/160/41/4
    1060/40/40/40/40/160/42/4
    Single mouse (104)0/10/10/10/10/40/11/1
        Total0/130/130/130/130/520/134/13
MG002 (guaB; cp9 cp32-3)
    1020/40/40/40/40/160/40/4
    1040/40/40/40/40/160/40/4
    1060/40/40/40/40/160/41/4
    Single mouse (104)0/10/10/10/10/40/10/1
        Total0/130/130/130/130/520/131/13
MG024 (fliG-1; no plasmids lost)
    1020/40/40/40/40/160/40/4
    1040/40/40/40/40/160/40/4
    1060/40/40/40/40/160/40/4
    Single mouse (104)0/10/10/10/10/40/10/1
        Total0/130/130/130/130/520/130/13
MG009 (BB0743; cp9)
    1020/41/41/42/44/162/42/4
    1041/44/44/43/412/164/44/4
    1060/44/43/44/411/164/44/4
    Single mouse (104)0/11/11/11/13/41/11/1
        Total1/1310/139/1310/1330/5211/1311/13
MG013 (insertion site ND; cp9)
    1020/41/40/41/42/161/40/4
    1040/42/41/43/46/163/44/4
    1062/44/40/44/410/164/44/4
    Single mouse (104)0/11/10/11/12/41/11/1
        Total2/138/131/139/1320/529/139/13
MG014 (mutS; cp9, cp32-6)
    1020/40/40/40/40/160/40/4
    1042/43/42/43/410/163/43/4
    1061/44/43/44/412/164/44/4
    Single mouse (104)0/11/11/11/13/41/11/1
        Total3/138/136/138/1325/528/138/13
MG019 (BBC08; lp38)
    1020/40/40/40/40/160/40/4
    1040/41/40/41/42/161/40/4
    1060/42/41/40/43/162/42/4
    Single mouse (104)0/10/11/11/12/41/11/1
        Total0/133/132/132/137/524/133/13
  • a Transposon insertion sites and plasmids missing (in addition to lp28-4 and lp56, which are absent in the parental strain 5A18 NP1) are indicated for each clone. Single mouse, results from single mouse inoculation study (see Table 1). ND, not determined.

  • b χ2 analysis found the combined 104 and 106 inoculum data for each of the mutants to be statistically different from those for the 5A18 NP1 control (P < 0.05).

  • c Reactive mice are those for which a significant serological response was demonstrated in a VlsE ELISA.