TABLE 1.

Insertion sites of pMarGent mutants (grouped by infectivity phenotype)

InfectivityaCloneReplicon/positionbInsertion site(s)cDescription
+MG004Chrom./108542BB0110 (108307-109671)Hypothetical protein
+MG005cp32-6/24624BBM36 (24619-25044)Borrelia plasmid protein B (bppB) (putative)
+MG006lp36/17582BBK25 (17565-16966) promoterTransposase-like protein (putative), authentic frameshift
+MG007Chrom./534145Intergenic; BB0524 (534301-535115) and BB0523 (533980-534072)BB0524 possible myoinositol-1 (or 4)- monophosphatase (putative); BB0523 is a hypothetical protein
+MG010cp32-3/27222BBS41 (26708-27298)Outer surface protein G (erpG)
+MG011lp28-3/4210BBH09 (7728-3892)Hypothetical protein
+MG012lp28-1/4515BBF10 (4488-4985)Hypothetical protein
+MG016cp32-8/25599BBL38 (25951-25175)Borrelia plasmid protein C (bppC) (putative)
+MG017lp38/33790Intergenic; BBJ45 (32498-33220) and BBJ46 (34668-34372)BBJ45 and BBJ46 are hypothetical proteins
+MG018cp32-4/24618BBR37 (24210-24635)Borrelia plasmid protein B (bppB) (putative)
+MG020cp26/23390BBB28 (23255-24499)Hypothetical protein
+MG025lp21/7829Intergenic; BBU05 (2868-3656) and BBU06 (14633-15235)Within central 63-bp repeat region of lp21
+MG026lp36/23615BBK37 (23953-22910)Homolog of immunogenic protein P37
+MG027cp32-4/7055BBR11 (6711-7823)Hypothetical protein
+MG028cp32-4/17887BBR29 (18664-17573)Conserved hypothetical protein
+MG029lp28-1/5088Intergenic; BBF10 (4488-4985) and BBF11 (5435-5542)BBF10 and BBF11 are hypothetical proteins
+MG030cp26/25676BBB29 (24825-26453)Phosphotransferase system enzyme, maltose- and glucose-specific IIABC component (malX)
+MG032lp17/9413Intergenic; BBD14 (8269-9381) and BBD15 (10015-9593)BBD14 is a conserved hypothetical protein and BBD15 is a hypothetical protein
+/− (lp28-1)MG008Chrom./660583BB0629 (661524-659644)Phosphotransferase system enzyme II (fruA-2)
+/− (lp28-1)MG015lp38/25996BBJ34 (26407-25337)Hypothetical protein
+/− (lp28-1)MG021lp28-2/20360BBG24 (22310-19620)Hypothetical protein
+/− (lp28-1)MG022lp25/21344Intergenic; BBE29 (19697-20886) and BBE30 (21558-21704)BBE29, putative adenine-specific methyltransferase, authentic frameshift; BBE30, hypothetical protein
+/− (lp28-1)MG023lp21/6922Intergenic; BBU05 (2868-3656) and BBU06 (14633-15235)Within central 63-bp repeat region of lp21
+/−(lp28-1)MG031cp26/12701BBB16 (12014-13606)Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (oppAIV)
+/− (lp28-1+)MG009Chrom./786112BB0743 (785057-786754)Hypothetical protein
+/− (lp28-1+)MG013Not determined
+/− (lp28-1+)MG014Chrom./841814BB0797 (839244-841832)DNA mismatch repair protein (mutS)
+/− (lp28-1+)MG019cp9/5623BBC08 (5534-5980)Conserved hypothetical protein
+/− (lp28-1+)MG033cp32-7/22694BBO35 (22755-22627)Hypothetical protein
MG001Not determined
MG002cp26/14424BBB17 (15107-13893)IMP dehydrogenase (guaB)
− (lp25)MG003cp26/11461Intergenic between BBB14 (11417-10920) and BBB15 (11636-11740)BBB14 and BBB15 are hypothetical proteins
MG024Chrom./226987BB0221 (227836-226610)Flagellar motor switch protein (fliG-1)
  • a +, culture positive at all sites; +/−, culture positive at some sites; −, culture negative at all sites.

  • b Chrom., chromosome.

  • c Position of gene given in parentheses. Reverse coordinates indicate that the gene is encoded on the negative strand.