TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype or relevant characteristicsDerivation or source
Strains
    E. coli
        χ6212F λ φ80 Δ(lacZYA-argF) endA1 recA1 hsdR17 deoR thi-1 glnV44 gyrA96 relA1 ΔasdA4Lab collection
        χ7213thi-1 thr-1 leuB6 fhuA21 lacY1 glnV44 ΔasdA4 recA1 RP4 2-Tc::Mu [λpir]; KmrLab collection
        LMG194F ΔlacX74 galE thi rpsL ΔphoA (PvuII) Δara714 leu::Tn10Invitrogen
    Salmonella serovar Typhimurium
        χ3761UK-1 wild typeLab collection
        χ8879ΔasdA16 ΔphoP233 ΔsptP1033::xylEThis study
        χ9085ΔasdA16 ΔphoP233 ΔsptP1033::xylE hisG atrB13::mudJχ8879
        χ8916ΔasdA16 ΔphoP233Lab collection
        χ9086ΔasdA16 ΔphoP233 hisG atrB13::mudJχ8916
Plasmids
    pYA3653Plasmid Asd+; p15AoriThis study
    pYA3657566-bp DNA encoding EASZ240 in pYA3653This study
    pYA3658792-bp DNA encoding EAMZ250 in pYA3653This study
    pYA3620β-Lactamase signal sequence-based periplasmic secretion plasmidLab collection
    pYA3731566-bp DNA encoding EASZ240 in pYA3620This study
    pYA3696566-bp DNA encoding EASZ240 in pBAD/HisC; AprThis study
    pYA3697792-bp DNA encoding EAMZ250 in pBAD/HisC, AprThis study