TABLE 4.

Growth promotion test of E. coli strainsa

SubstrateGrowth of strain
i484fitA-(xy006)fitB-(xy005)fhuA-(xy007)fepC-(xy008)fepA-(xy009)AA93/ pfit1AA93/ pCR
Si484++++++++
SAB1515++++++
Heme+++NDNDND
Heme-BSA+++NDNDND
DHBA+++NDNDND
LactoferrinNDNDND
TransferrinNDNDND
Desferal+++NDNDND
RA+++NDNDND++
Ferrichrome+++NDND++
Iron citrate+++NDNDND
FeSO4++++++++
FeCl3++++++++
dH2O
  • a Concentrations of iron compounds used are reported in Materials and Methods. Si484 and SAB1515 represent supernatants of E. coli i484 and AB1515.199 cells, respectively, grown in iron-restricted media. Heme-BSA was made by mixing 100 μM heme and 100 μM BSA at a 1:1 molar ratio. Iron citrate was made by mixing fresh FeSO4 with sodium citrate at a molar ratio of 1:1,000. +, positive; −, negative; ND, not determined; DHBA, dihydroxybenzoic acid; dH2O, distilled water.