TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionSource or reference
E. coli strains
    i484O25:H autoagluttinating; human isolate18
    xy006i484, fitA::EZ-TN5 <Kan-2>This study
    xy005i484, fitB::EZ-TN5 <Kan-2>This study
    xy009i484, fepA::EZ-TN5 <DHFR>This study
    xy008i484, fepC::EZ-TN5 <DHFR>This study
    xy007i484, fhuA::EZ-TN5 <DHFR>This study
    oy016i484, lacZ::EZ-TN5 <DHFR>This study
    AA93F araD139 ΔlacU169 rpsL150 relA1 deoC1 flbB5301 ptsF25 rbsR aroB fecB::Mud1 (Ap lac)26
    oy097AA93; feoB::EZ-TN5 <DHFR>This study
    AB1515.199purE42 proC14 leu-6 trpE38 thi-1 fhuA23 lacY1 (fepC::Tn5)6
    123Field isolatePig
    124Field isolatePig
    252Field isolatePig
    EcoR33Field isolatePig
    263Field isolatePig
    431Field isolatePig
    987Field isolatePig
    1413Field isolatePig
    16Field isolateHuman
    17Field isolateHuman
    18Field isolateHuman
    EcoR40Field isolateHuman
    EcoR60Field isolateHuman
    EcoR62Field isolateHuman
    EcoR8Field isolateHuman
    EcoR26Field isolateHuman
    EcoR42Field isolateHuman
    EcoR45Field isolateHuman
    DH5αF φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdS17 (rK mK+) deoR thi-1 supE44 λ gyrA96 relA1Invitrogen
Plasmids
    pMP220Contains a promoterless lacZ gene, Tetr IncP33
    pMP-fitAfit promoter cloned into pMP220 with direction of fitA transcriptionThis study
    pMP-fitBfit promoter cloned into pMP220 with direction of fitB transcriptionThis study
    pCRCloning plasmid pCR-XL-TOPOInvitrogen
    pCR63lacZ cloned into pCR-XL-TOPOThis study
    pCR64fitR cloned into pCR-XL-TOPOThis study
    pfit1Whole fit region cloned into pCR-XL-TOPOThis study