TABLE 2.

Isolation of RASV strain from chicken tissues at various times following inoculation

Plasmida and no. of days after inoculationLog10 colony count of RASV strain isolated per g of tissue (geometric mean ± SE)b
LiverSpleenBursaCecum
pYA3653
    32.13 ± 2.17c2.26 ± 2.24c6.81 ± 0.638.42 ± 0.09
    64.03 ± 1.775.57 ± 1.016.74 ± 0.638.58 ± 0.85
    93.68 ± 2.046.24 ± 0.566.30 ± 0.888.89 ± 0.36
    122.67d4.43d6.71 ± 0.369.94 ± 1.44
    152.1d2.37d5.06 ± 0.277.54 ± 0.49
pYA3657
    3NANA6.73 ± 1.18.56 ± 0.32
    62.71 ± 0.174.81 ± 0.665.99 ± 0.218.48 ± 0.47
    91.72 ± 1.58c3.34 ± 2.90c5.39 ± 1.049.20 ± 0.38
    121.82d2.05 ± 1.97c5.75 ± 0.558.10 ± 0.84
    153.82d2.43 ± 2.30c6.13 ± 0.308.68 ± 0.74
pYA3658
    32.08 ± 1.80c2.08 ± 2.93c6.48 ± 0.528.03 ± 0.37
    62.69 ± 2.69c4.92 ± 1.415.75 ± 0.777.70 ± 0.11
    93.54 ± 1.654.24 ± 3.70c6.12 ± 1.289.55 ± 0.75
    120.00 ± 0.003.72 ± 3.22c5.77 ± 0.429.11 ± 0.14
    154.33d3.43 ± 3.22c5.31 ± 0.699.19 ± 0.82
  • a Groups of 15 chicks were inoculated on the day of hatching with Salmonella serovar Typhimurium χ8879 (ΔsptP1033::xylE ΔasdA16 ΔphoP233) harboring plasmid pYA3653, pYA3657, or pYA3658.

  • b Obtained from three chickens. NA, data not available.

  • c One chick had <10 CFU/g of tissue.

  • d Two chicks had <10 CFU/g of tissue.