TABLE 3.

Polypeptides unique or up-regulated in the PrfA L140F secretome

Functional classGene designationProtein nameProtein descriptionSpot no.Peptide matchaCoverage (%)bPredicted pIPredicted mass (kDa)Observed pIObserved mass (kDa)Reference(s) or source for virulence contribution
Virulencelmo0202LLOListeriolysin O912472.08.258.77.760.038,50
lmo0204ActAActin assembly-16046.34.770.35.9125.014,40
    inducing protein24826.65.680.0
111926.05.537.0
lmo0205PlcBPhospholipase C13-17723.58.433.35.830.065
lmo1786InlCInternalin C21927.76.533.15.830.015,21
ABC transporterlmo0135Similar to oligopeptide ABC transport system substrate-binding proteins84053.64.858.35.958.0This study
lmo2196OppASimilar to pheromone ABC transporter (binding protein)33049.65.162.66.080.04
lmo2417Conserved lipoprotein (putative ABC transporter binding protein)13-17415.65.130.75.830.0
Cell walllmo1438Similar to penicillin-binding protein51017.58.379.96.285.529
    modifying63143.06.385.5
73651.55.883.0
lmo2522Similar to hypothetical cell wall binding protein from B. subtilis13-17615.95.829.15.830.0
17523.16.514.0
203363.95.730.5
lmo2691MurA or NamASimilar to autolysin, N-acetylmuramidase327.110.363.66.080.09,42
3 and 4714.76.078.0
lmo2754PBP5Similar to d-alanyl-d-alanine carboxypeptidase (penicillin-binding protein 5)122042.57.648.16.845.029
Antigenic lipoproteinlmo1388TcsACD4+ T-cell-stimulating (surface) antigen, lipoprotein101859.44.838.45.335.0This study
lmo2637Conserved lipoprotein (putative)182236.16.632.76.033.0
19210.75.732.0
Secretionlmo0292HtrASimilar to heat shock protein HtrA serine protease2821.64.352.85.680.062,70
lmo2219PrsA2Similar to posttranslocation molecular chaperone (protein export protein) (PrsA from B. subtilis)191339.25.632.75.732.010, this study
201743.75.631.0
  • a Number of tryptic peptides detected that could be matched to each protein.

  • b Amino acid coverage calculated from the whole protein.