Table 2.

Clinical and histological findings in calves 18 and 42 h after inoculation with an eae+ EHEC strain [86-24 or 86-24eaeΔ10(pEB310)], aneae mutant EHEC strain (86-24eaeΔ10), or a strain lacking the eae gene (B2F1 or nonpathogenic E. coli 123)

StrainTime of determination (h postinoculation)nNo. positive for:
DiarrheaDeathA/E bacteria ina:
RectumColonCecumIleum
86-24182202222
42331b3322
86-24eaeΔ10183000000
422000000
86-24eaeΔ10(pEB310)183103323
42431b3333
B2F1423000000
123423000000
  • a A/E bacteria stained with E. coli O157:H7 antibody by immunoperoxidase technique.

  • b Calf found dead at ca. 40 h postinoculation; samples were collected for histopathological studies.