Table 5.

Characterization of transposon mutants

CosmidSite of insertionaImmuno- reactivityb
DH5αSφ874
pTEX5159G orfde4
pTEX5159D orfde4
pTEX5159H
pTEX5159I orfde5
pTEX5159E orfde5 DNGe
pTEX5159J orfde5
pTEX5159K orfde5
pTEX5159F orfde5 DNG
pTEX5159L orfde4/5
pTEX5159M
pTEX5159A orfde3 ++
pTEX5159B orfde8 +
pTEX5159C orfde3-orfde4 ++
pTEX5159Nc r7, 0.7 kb upstream of contig C++
pTEX5159Oc orfde2 ++
pTEX5159Qc r7, 0.2 kb upstream of contig C++
pTEX5159Pc r7, 0.7 kb upstream of contig C++
pTEX5159Rc orfde2 ++
pTEX5159Sc r6, 1.8 kb downstream of contig C+−?d
pTEX5159Tc r6, 1.8 kb downstream of contig C+d
pTEX5159Uc r6, 1.9 kb downstream of contig C+d
  • a ORF where the transposon had inserted. Sites of insertion of those that were not sequenced were estimated based on their PCR product sizes in relation to the ones that were sequenced.

  • b Tested by using colonies of each clone in immunoblot analyses with patient serum.

  • c Not been subjected to PCR amplification. The insertion site was determined by RED analysis.

  • d The clone was transformed into Sφ874, resulted in large deletions in the insert, and was immunonegative.

  • e Sφ874 transformants of pTEX5159E and pTEX5159F did not grow (DNG) when streaked from the primary transformation plates.