Table 2.

Detection of intestinally derived transductants at 24 h

Donor strainaMouseNo. of cells in stool (CFU/ml)bTransductant ratiof
RecipientcDonordTransductantse
MC4100 H-19B-Ap116.2 × 1052.1 × 1062.0 × 1029.5 × 10−5
28.8 × 1061.1 × 1074.2 × 1033.8 × 10−4
34.4 × 1064.8 × 1068.0 × 1021.7 × 10−4
42.2 × 1069.1 × 1053.8 × 1014.2 × 10−5
MC4100 H-19B-Ap257.6 × 1065.8 × 1044.0 × 1016.8 × 10−4
66.8 × 1068.9 × 1042.1 × 1022.3 × 10−3
  • a Approximately 1 × 109MC4100 H-19B-Ap1 cells or 4 × 106 MC4100 H-19B-Ap2 cells were inoculated intragastrically as donors. Approximately 109 MC4100 srl::Tn5(Knr) cells were intragastrically inoculated 45 min later as potential recipients.

  • b Individual pellets were resuspended in 1 ml of Luria broth.

  • c The recipient cell number was determined by counting the number of Smr KnrLacZ CFU.

  • d The donor cell number was determined by counting the number of Smr AprLacZ CFU.

  • e The number of transductants was determined by counting the number of Smr Knr AprLacZ CFU. The assay detection limit is 10 CFU/ml.

  • f Calculated by dividing the number of transductants by the number of donor cells.